After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE. 定量后,分别进行一维等电聚焦分离和二维聚丙烯酰胺凝胶电泳分离。
Methods: in the present experiment, acid dehydroxy amino acid analysis and isoelectric focusing in polyacylamide gels were used and the amino acid composition and isoelectric point of porcine platelet-derived growth factor were studied. 方法:用酸水解及聚丙烯酰胺凝胶等电聚焦的方法测定猪血小板衍生生长因子的氨基酸含量、组成成分和等电点。
Isoelectric Focusing and Immunoblotting Identification of Human ApoE Phenotype 人血清apoE表型等电聚焦免疫印迹分析法
In this study, isoelectric focusing electrophoresis program was optimized, and various sample preparation methods, different loading quantities and strip length were studied. 在实验中,优化了等电聚焦程序,研究了精子蛋白的不同制备方法、不同上样量、不同胶条长度对电泳结果的影响。
Microchip free flow isoelectric focusing with immobilized pH gradient on monolithic materials 基于固定化pH梯度整体材料的芯片自由流等电聚焦电泳模式的构建
Isoelectric focusing may be used as another indication. 等电聚焦可以用为另一指标。
Isoelectric focusing A technique used in ELECTROPHORESIS to separate amphoteric molecules ( able to combine with either acids or bases). 等电点聚焦:是一种利用电泳技术分离两性分子(能与酸或碱结合)的技术。
Analysis of Malate Dehydrogenase Isozymes in Taihe Negro Fowl by Thin layer Isoelectric Focusing Polyacrylamide Gel Electrophoresis 水平聚焦-电离层的泰和乌骨鸡苹果酸脱氢酶同工酶的薄层等电聚焦电泳分析
Isoelectric focusing of STFC in thin-layer polyacrylamide gel obtained only one band as shown by Coomassie Blue staining. 在等电点聚焦分析中该成分为一条考马斯亮蓝染色带。
The tablecloth trails on the floor. Isoelectric Focusing and Immunoblotting Identification of Human ApoE Phenotype 台布一直垂到地板上。人血清apoE表型等电聚焦免疫印迹分析法
By using RAPD technique and isoelectric focusing electrophoresis method, genomic DNA and protein from Guangdong strain and Fujian strain of Echinochasmus fujianensis were analysed respectively. 采用RAPD技术和等电聚焦电泳方法,对福建棘隙吸虫福建株和广东株虫体进行基因组DNA多态性分析和蛋白质等电点测定。
BSA and Hemoglobin Separation by Pressure-Driven Capillary Isoelectric Focusing 压力驱动的毛细管等电聚焦分离牛血清白蛋白和血红蛋白
Studies on Proteins of Foot Muscle of Oncomelania Snail Separated by Disc Electrophoresis and Isoelectric Focusing 用盘电泳和等电聚焦方法分离钉螺足肌蛋白质的研究
Study of Several Isoenzyme on Eggplant Seeds Using the Isoelectric Focusing Method 用等电聚焦法对茄子种子几种同工酶的研究
On line coupling of capillary isoelectric focusing ( CIEF) to electrospray ionization mass spectrometry ( ESI MS) as a two dimensional separation/ analysis system was employed for high resolution analysis of the protein isoforms observed during CIEF process. 在线的毛细管等电聚焦电喷雾质谱联用,作为一种二维的分离系统,对毛细管等电聚焦过程中形成的蛋白质亚型进行了分析。
Advances in Capillary Isoelectric Focusing Electrophoresis 毛细管等电聚焦电泳技术进展
The results showed that low ion intensity sample washing buffer improved the performance of isoelectric focusing. 结果表明:采用低离子强度的缓冲液冲洗收集样品可以提高等电聚焦效果。
Capillary isoelectric focusing ( CIEF) is a more rapid technique for separating proteins that the traditional gel-based technique. 和传统的凝胶技术相比,毛细管等电聚焦(CIEF)是一种蛋白质的快速分离技术。
Polyacrylamide Gel Isoelectric Focusing and Electrophoresis for CSF Proteins 脑脊液蛋白聚丙烯酰胺凝胶等电聚焦电泳
Strategy for Protein Mixture Identification by Immobilized pH Gradients Isoelectric Focusing and Mass Spectrometry 一维固相pH梯度等电聚焦与生物质谱联用鉴定混合蛋白质的方法建立与应用
Study on the isoelectric point of low temperature induced protein of straw mushroom measured by isoelectric focusing electrophoresis 等电聚焦电泳测定草菇低温诱导蛋白等电点的研究
Capillary isoelectric focusing ( CIEF) can separate proteins with pls different from each other by 0.02 pH unit. 毛细管等电聚焦(CIEF)可分离等电点(pI)相差0.02pH单位的蛋白质。
Separation of proteins by capillary isoelectric focusing and electroosmotic pump driving 毛细管等电聚焦和电渗泵驱动聚焦区带分离蛋白质
Methods: A 2-DE technique, including isoelectric focusing ( IEF) with an immobilized pH gradient ( IPG) and SDS-PAGE, was used. Sample preparation, rehydration, SDS-PAGE and other steps were established and optimized. 方法:采用固相pH梯度(IPG)等电聚焦(IEF)为第一向、SDS-PAGE垂直电泳为第二向的双向电泳技术,对样品的处理、水化、等电聚焦、SDS-PAGE等步骤进行了优化。
Inheritance of PGI isozymes in chestnut species was analyzed using isoelectric focusing on thin layer polyacrylamide slab gels and single tree progeny method. 采用超薄聚丙烯酰胺平板凝胶等电聚焦电泳和单株后代法,分析了栗属种的PGI同工酶的遗传。
Protein analysis of Cysticercus cellulosae with polyacrylamide gel and isoelectric focusing electrophoresis 猪囊尾蚴蛋白质的聚丙烯酰胺凝胶电泳和等电聚焦电泳分析
Isoelectric focusing overlay technique and conjugation experiments were used to find ESBLs and determine the phenotypical characteristics of ESBLs. 通过等电聚焦电泳和接合试验分析ESBLs的表型特征;
Molecular mass of samples were assayed by mass spectrometry, and isoelectric point was identified by isoelectric focusing. 质谱测定样品分子量,等电聚焦测定等电点进行鉴定。